Effect of rhizosphere bacteria on antioxidant enzymes and some biochemical characteristics of Medicago sativa L. subjected to herbicide stress

Abstract Abiotic stresses such as herbicides can affect plant growth and yield. Using herbicide-resistant growth-promoting bacteria is a new approach to mitigate these side effects. This study was conducted evaluate the effect of three native isolated from Medicago sativa rhizosphere, including Serratia rubidaea (A) Pseudomonas putida (B), sp. (C) plus Synorhizobium meliloti (R) their combinations (AB, AC, BC, ABC, AR, BR, CR, ABR, ACR, BCR, ABCR) on microbial population, biomass, antioxidant enzymes (CAT, APX, GPX) activities, hydrogen peroxide malondialdehyde contents at presence absence imazethapyr herbicide. The results indicated that herbicide application decreased biomass but increased concentrations all inoculated non-inoculated plants. Bacterial inoculation in most cases activities. These increases were more evident under application. highest increase attributes achieved by AB, ABR inoculums activities ABCR inoculations. It be concluded addition promotion, resistance against stress controlling free-radical induced oxidative lipid peroxidation through enzymes. findings create visions biofertilizer preparation for reducing environmental stresses.